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Kunkee Medium

[所屬分類:培養基配方] [發布時間:2021-11-24] [發布人:網站管理員2] [閱讀次數:] [返回]
Kunkee Medium

山東(dong)拓普生物(wu)工(gong)程有限公司 培養基配方 //ljxinglong.com

Composition per 1000.88mL:

Pancreatic digest of casein................................20.0g
Glucose ...............................................................5.0g
Peptone................................................................5.0g
Yeast extract........................................................5.0g
Filtered tomato juice ................................... 250.0mL
Ethanol (96% solution) ............................... 176.0mL
Tween 80......................................................... 0.5mL
pH 5.5 ± 0.2 at 25°C
Preparation of Medium: Add components, ex
cept ethanol, to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Distribute into tubes
in 5.0mL volumes. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to room temperature. Asepti
cally add 0.88mL of ethanol solution to each tube con
taining 5.0mL of medium.
Use: For the cultivation of Lactobacillus fruc
tivorans, which has been isolated from spoilt sake.
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