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培養基配方
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Kleb Agar (m-Kleb Agar)
[所屬分類:培養基配方] [發布時間:2021-11-24] [發布人:網站管理員2] [閱讀次數:] [返回]
Kleb Agar
(m-Kleb Agar)
山東(dong)拓普生物工程有限(xian)公(gong)司 培(pei)養基配方 //ljxinglong.com
Composition per liter:
Agar ..................................................................15.0g
Proteose peptone No. 3 .....................................10.0g
NaCl....................................................................5.0g
Adonitol ..............................................................5.0g
Beef extract.........................................................1.0g
Aniline Blue........................................................0.1g
Sodium lauryl sulfate..........................................0.1g
Phenol Red......................................................0.025g
Ethanol (95% solution) ................................. 20.0mL
Carbenicillin solution.................................... 10.0mL
pH 7.4 ± 0.2 at 25°C
Carbenicillin Solution:
Composition per 10.0mL:
Carbenicillin......................................................0.05g
Preparation of Carbenicillin Solution: Add
carbenicillin to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept ethanol and carbenicillin solution, to distilled/
deionized water and bring volume to 970.0mL. Mix
thoroughly. Gently heat and bring to boiling. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Aseptically add 20.0mL of ethanol and
10.0mL of carbenicillin solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the enumeration of bacteria from waters.
