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King’s B Medium (BAM M69)

[所屬分類:培養基配方] [發布時間:2021-11-24] [發布人:網站管理員2] [閱讀次數:] [返回]
King’s B Medium
(BAM M69)

山東拓(tuo)普生(sheng)物工(gong)程(cheng)有(you)限(xian)公司 培養(yang)基配方 //ljxinglong.com

Composition per liter:

Proteose peptone No. 3 .....................................20.0g
Agar ................................................................150.0g
K2HPO4, anhydrous ............................................1.5g
MgSO4·7H2O ......................................................1.5g
Glycerol ........................................................ 10.0mL
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Distrib
ute into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes or leave
in tubes.
Use: For the nonselective isolation, cultivation, and
pigment production of Pseudomonas species.
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