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KC Broth

[所屬分類:培養基配方] [發布時間:2021-11-24] [發布人:網站管理員2] [閱讀次數:] [返回]
KC Broth

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................10.0g
KCl......................................................................5.0g
CaCl2 solution................................................. 0.5mL
CaCl2 Solution:
Composition per 10.0mL:
CaCl2·2H2O.......................................................1.47g
Preparation of CaCl2 Solution: Add 1.47g of
CaCl2·2H2O to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept CaCl2 solution, to distilled/deionized water and
bring volume to 999.5mL. Mix thoroughly. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 25°C. Aseptically add 0.5mL
of CaCl2 solution. Mix thoroughly. Aseptically distrib
ute into sterile tubes.
Use: For the cultivation of Escherichia coli.
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