培養基配方

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Kanamycin Esculin Azide Agar

[所屬分類:培養基配方] [發布時間:2021-11-24] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓(tuo)普(pu)生物(wu)工程有限公司培(pei)養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................20.0g

Agar ..................................................................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................5.0g

Esculin ................................................................1.0g

Sodium citrate.....................................................1.0g

Ferric ammonium citrate.....................................0.5g

NaN3 .................................................................0.15g

Kanamycin sulfate solution .......................... 10.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Kanamycin Sulfate Solution:

Composition per 10.0mL:

Kanamycin sulfate .........................................20.0mg

Preparation of Kanamycin Sulfate Solution:

Add kanamycin sulfate to distilled/deionized water

and bring volume to 10.0mL. Mix thoroughly.

Caution: Sodium azide is toxic. Azides also react

with metals and disposal must be highly diluted.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Distrib

ute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C. Pour into sterile Petri dishes or leave

in tubes.

Use: For the isolation of enterococci from foods.

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