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培養基配方

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J Agar

[所屬分類:培養基配方] [發布時間:2021-11-24] [發布人:網站管理員2] [閱讀次數:] [返回]

J Agar

山東拓普生(sheng)物工程有限公司培(pei)養基配(pei)方 //ljxinglong.com

Composition per liter:

Agar ..................................................................20.0g

Yeast extract......................................................15.0g

Pancreatic digest of casein..................................5.0g

K2HPO4...............................................................3.0g

Glucose solution ........................................... 10.0mL

pH 7.3–7.5 at 25°C

Glucose Solution:

Composition per 10.0mL:

Glucose ...............................................................2.0g

Preparation of Glucose Solution: Add glucose

to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept glucose solution, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptical

ly add sterile glucose solution. Mix thoroughly. Pour

into sterile Petri dishes.

Use: For the cultivation of Bacillus species and

Sporolactobacillus species.

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