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培養基配方
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Iron Milk Medium
[所屬分類:培養基配方] [發布時間:2021-11-24] [發布人:網站管理員2] [閱讀次數:] [返回]
Iron Milk Medium
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Iron filings...........................................................1.0g
Whole milk ........................................................ 1.0L
pH 6.8 ± 0.2 at 25°C
Preparation of Medium: Add iron filings, which
may be small balls of steel wool, to whole milk and
bring volume to 1.0L. Mix thoroughly. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C.
Use: For the cultivation of lactic acid bacteria. For
the cultivation and differentiation of Clostridium
species. The medium turns black if H2S is produced.
The medium turns red if acid is produced from milk
carbohydrate fermentation. Acid and gas production
are characteristic of Clostridium perfringens and
Clostridium butyricum.



