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山東拓普生物工程有限(xian)公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anthranilic Acid Medium, Revised
Composition per 1040.0mL:
Na 2 HPO 4 .......................................................................................6.0g
KH 2 PO 4 .........................................................................................3.0g
NH 4 Cl ...........................................................................................1.0g
NaCl..............................................................................................0.5g
Glucose solution......................................................................25.0mL
CaCl 2 solution..........................................................................10.0mL
MgSO 4 solution .......................................................................10.0mL
Anthranilic acid solution............................................................5.0mL
Glucose Solution:
Composition per 100.0mL:
D- Glucose....................................................................................20.0g
Preparation of Glucose Solution: Add  D- glucose to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
CaCl 2 Solution:
Composition per 100.0mL:
CaCl 2 ·2H 2 O ..............................................................................0.147g
Preparation of CaCl 2 Solution: Add CaCl 2 to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
MgSO 4 Solution:
Composition per 10.0mL:
MgSO 4 ·7H 2 O..............................................................................2.47g
Preparation of MgSO 4 Solution: Add MgSO 4 ·7H 2 O to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Anthranilic Acid Solution:
Composition per 100.0mL:
Anthranilic acid ............................................................................1.0g
Ethanol (95% solution)..........................................................100.0mL
Preparation of Anthranilic Acid Solution: Add anthranilic acid
to 100.0mL of ethanol. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except glucose solu-
tion, CaCl 2 solution, MgSO 4 solution, and anthranilic acid solution, to
distilled/deionized water and bring volume to 990.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 25.0mL of sterile
glucose solution, 10.0mL of sterile CaCl 2 solution, 10.0mL of sterile
MgSO 4 solution, and 5.0mL of sterile anthranilic acid solution. Mix
thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation of Escherichia coli.