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HiCrome E. coli Agar B (E. coli Agar B, HiCrome)

[所屬分類:培養基配方] [發布時間:2021-11-22] [發布人:網站管理員2] [閱讀次數:] [返回]
HiCrome E. coli Agar B
(E. coli Agar B, HiCrome)

山東拓(tuo)普(pu)生(sheng)物工程(cheng)有限公(gong)司(si) 培(pei)養基配方 //ljxinglong.com

Composition per liter:

Casein enzymic hydrolysate .............................20.0g
Agar ..................................................................15.0g
Bile salts mixture ................................................1.5g
X-Glucuronide ................................................0.075g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available from Fluka, Sig
ma-Aldrich.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat while stirring and bring
to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Pour into sterile Petri dishes.
Use: For the detection and enumeration of Escheri
chia coli in foods without further confirmation on
membrane filter or by indole reagent. The chro
mogenic agent X-glucuronide used in this medium
helps to detect glucuronidase activity. E. coli cells
absorb X-glucuronide and the intracellular glucu
ronidase splits the bond between the chromophore
and the glucuronide. The released chromophore
gives coloration to the colonies. Bile salts mixture in
hibits gram-positive organisms.
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