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Ham’s F-10 Medium (BAM M58)

[所屬分類:培養基配方] [發布時間:2021-11-22] [發布人:網站管理員2] [閱讀次數:] [返回]
Ham’s F-10 Medium
(BAM M58)

山(shan)東拓普生(sheng)物工程(cheng)有限公(gong)司(si) 培養基(ji)配(pei)方(fang) //ljxinglong.com

Composition per liter:

NaCl....................................................................7.4g
NaHCO3..............................................................1.2g
Glucose ...............................................................1.1g
NaH2PO4·H2O...................................................0.29g
KCl....................................................................0.28g
L-Arginine·HCl..................................................0.21g
L-Glutamine ......................................................0.15g
MgSO4·7H2O....................................................0.15g
Sodium pyruvate...............................................0.11g
KH2PO4.............................................................0.08g
CaCl2·2H2O.......................................................0.04g
L-Cystine·2HCl..................................................0.04g
L-Histidine·HCl·H2O.........................................0.02g
L-Lysine·HCl .....................................................0.02g
L-Asparagine-H2O.............................................0.01g
L-Aspartic Acid .................................................0.01g
L-Glutamic acid.................................................0.01g
L-Leucine...........................................................0.01g
L-Proline............................................................0.01g
L-Serine .............................................................0.01g
L-Alanine..........................................................8.9mg
Glycine.............................................................7.5mg
D-Phenylalanine ...............................................5.0mg
L-Methionine ....................................................4.5mg
Hypoxanthine...................................................4.1mg
L-Threonine ......................................................3.6mg
L-Valine ........................................................... 3.5mg
L-Isoleucine ......................................................2.6mg
L-Tyrosine.........................................................1.8mg
Vitamin B12 ......................................................1.4mg
Folic acid..........................................................1.3mg
Phenol Red.......................................................1.2mg
Thiamine·HCl ..................................................1.0mg
FeSO4·7H2O.....................................................0.8mg
Choline chloride...............................................0.7mg
D-Calcium pantothenate ...................................0.7mg
Thymidine........................................................0.7mg
Niacinamide.....................................................0.6mg
L-Tryptophan....................................................0.6mg
Isoinositol.........................................................0.5mg
Riboflavin ........................................................0.4mg
Lipoic acid .......................................................0.2mg
Pyridoxine·HCl ................................................0.2mg
ZnSO4·7H2O ..................................................0.03mg
Biotin .............................................................0.02mg
CuSO4·5H2O .................................................... 3.0μg
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Filter sterilize.
Use: For the growth of Y-1 cell cultures used in the
mouse adrenal assay for heat-labile toxin of entero
toxigenic Escherichia coli and Vibrio species.
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