培養基配方

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Halophilic Broth (HB)

[所屬分類:培養基配方] [發布時間:2021-11-22] [發布人:網站管理員2] [閱讀次數:] [返回]

Halophilic Broth

(HB)

山東拓普生物工(gong)程(cheng)有限公(gong)司培養基配方 //ljxinglong.com

Composition per liter:

NaCl................................................................250.0g

MgSO4·7H2O ....................................................25.0g

Casamino acids .................................................10.0g

Yeast extract......................................................10.0g

Proteose peptone.................................................5.0g

Trisodium citrate.................................................3.0g

KCl......................................................................2.0g

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C.

Use: For the isolation and cultivation of halophilic

microorganisms from foods, such as Pseudomonas

species and Flavobacterium species from fish and

salted foods.