培養基配方

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Glucose Salts Medium

[所屬分類:培養基配方] [發布時間:2021-11-19] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 1000.5mL:

Glucose ...............................................................5.0g

(NH4)2SO4...........................................................1.0g

MgSO4·7H2O ......................................................0.5g

NaCl....................................................................0.5g

NaH2PO4·12H2O..............................................0.7mg

NaH2PO4·2H2O................................................0.3mg

Trace elements solution .................................. 0.5mL

pH 6.9 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

H3BO3 .................................................................0.3g

CoCl2·6H2O ........................................................0.2g

ZnSO4·7H2O .......................................................0.1g

MnCl2·4H2O .....................................................0.03g

Na2MoO4·2H2O ................................................0.03g

NiCl2·6H2O .......................................................0.02g

CuCl2·2H2O ......................................................0.01g

Preparation of Trace Elements Solution: Add

components to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept trace elements solution, to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Au

toclave for 15 min at 15 psi pressure–121°C. Asepti

cally add 0.5mL of sterile trace elements solution.

Mix thoroughly. Aseptically distribute into sterile

tubes or flasks.

Use: For the cultivation of a wide variety of bacteria

and fungi.