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Glucose Phosphate Broth

[所屬分類:培養基配方] [發布時間:2021-11-19] [發布人:網站管理員2] [閱讀次數:] [返回]
Glucose Phosphate Broth

山(shan)東(dong)拓普生物工程有限公(gong)司 培養基配(pei)方 //ljxinglong.com

Composition per liter:

Peptone .............................................................10.0g
K2HPO4...............................................................5.0g
Glucose ...............................................................5.0g
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add components, ex
cept glucose, to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Gently heat and
bring to boiling. Filter while hot through Whatman
filter paper. Cool to 25°C. Adjust pH to 7.5. Add 5.0g
of glucose. Mix thoroughly. Distribute into sterile
tubes or flasks. Autoclave for 10 min at 10 psi pres
sure–115°C.
Use: For the cultivation of a variety of nonfastidious
heterotrophic microorganisms.
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