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Gelatinase Test Medium

[所屬分類:培養基配方] [發布時間:2021-11-19] [發布人:網站管理員2] [閱讀次數:] [返回]
Gelatinase Test Medium

山東(dong)拓普生物工程有(you)限公司 培(pei)養(yang)基配方(fang) //ljxinglong.com

Composition per liter:

Gelatin.................................................................3.0g
ACES buffer .......................................................1.0g
Yeast extract........................................................1.0g
Charcoal, activated ...........................................0.15g
α-Ketoglutarate monopotassium salt..................0.1g
L-Cysteine·HCl·H2O (4% solution)................. 1.0mL
KOH (85% solution)....................................... 1.0mL
Fe4(P2O7)3 solution......................................... 1.0mL
pH 6.9 ± 0.2 at 25°C
L-Cysteine·HCl·H2O Solution:
Composition per 10.0mL:
L-Cysteine·HCl·H2O............................................0.4g
Preparation of L-Cysteine·HCl·H2O Solu
tion: Add L-cysteine·HCl·H2O to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Fe4(P2O7)3 Solution:
Composition per 10.0mL:
Fe4(P2O7)3.........................................................0.15g
Preparation of Fe4(P2O7)3 Solution: Add
Fe4(P2O7)3 to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add ACES buffer to
distilled/deionized water and bring volume to
899.0mL. Mix thoroughly. Gently heat to 50°C. Add
1.0mL of KOH solution. Mix thoroughly. Add char
coal, yeast extract, and α-ketoglutarate. Add 80.0mL
of distilled/deionized water to wash sides of flask.
Mix thoroughly. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 50°C. Aseptically add 10.0mL
of sterile cysteine solution and 10.0mL of sterile
Fe4(P2O7)3 solution. Mix thoroughly. Adjust pH to
6.9. Aseptically distribute into sterile screw-capped
tubes.
Use: For the cultivation and differentiation of gelati
nase-producing bacteria.
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