培養基配方

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Folic Acid Agar

[所屬分類:培養基配方] [發布時間:2021-11-19] [發布人:網站管理員2] [閱讀次數:] [返回]

Folic Acid Agar

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Noble agar........................................................ 15.0g

K2HPO4·3H2O ................................................... 1.2g

Folic acid ........................................................... 1.0g

KH2PO4.............................................................. 0.5g

Salts A............................................................ 5.0mL

Salts B............................................................ 1.5mL

Salts A:

Composition per 100.0mL:

MgSO4·7H2O..................................................... 1.0g

CaCl2·2H2O ....................................................... 0.1g

FeSO4·7H2O....................................................... 0.1g

Preparation of Salts A Solution: Add compo

nents to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly. Maintain for 3 days at

25°C to dissolve. Filter sterilize the supernatant.

Salts B:

Composition per 100.0mL:

MnSO4 ...............................................................0.1g

Na2MoO4 ...........................................................0.1g

Preparation of Salts B Solution: Add compo

nents to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept salts A solution and salts B solution, to distilled/

deionized water and bring volume to 994.5mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add 5.0mL of sterile salts A

solution and 1.5mL of sterile salts B solution. Mix

thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the cultivation and maintenance of Pseudo

monas species.