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Fluid Thioglycolate Medium

[所屬分類:培養基配方] [發布時間:2021-11-19] [發布人:網站管理員2] [閱讀次數:] [返回]
Fluid Thioglycolate Medium

山東拓普生物工(gong)程有限(xian)公司 培養基配方(fang) //ljxinglong.com

Composition per liter:

Pancreatic digest of casein............................... 15.0g
Glucose .............................................................. 5.5g
Yeast extract....................................................... 5.0g
NaCl................................................................... 2.5g
Agar ................................................................. 0.75g
L-Cystine ............................................................ 0.5g
Sodium thioglycolate......................................... 0.5g
Resazurin ........................................................1.0mg
pH 7.1 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. If medium becomes oxi
dized before use (resazurin turns red), heat in a boil
ing water bath to expel absorbed O2. Cool to 25°C.
Use: For the cultivation of anaerobic, microaero
philic, and aerobic microorganisms. For use in steril
ity testing of a variety of specimens.
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