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FLN Medium (Fluorescence Lactose Nitrate Medium)
[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]
FLN Medium
(Fluorescence Lactose Nitrate Medium)
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per liter:
Lactose.............................................................20.0g
Agar ................................................................. 15.0g
Proteose peptone No. 3.................................... 10.0g
KNO3 ................................................................. 2.0g
K2HPO4.............................................................. 1.5g
MgSO4·7H2O .....................................................1.5g
NaNO2................................................................0.5g
Phenol Red.......................................................0.02g
pH 7.2 ±0.2 at 25°C
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dish
es or leave in tubes.
Use: For the differentiation of pseudomonads from
other nonfermentative bacilli. Lactose fermentation is
indicated by the medium turning yellow. Pseudomo
nas cepacia often produces acid from lactose. Denitri
fication from nitrate or nitrite is indicated by the
formation of gas bubbles in the solid medium.
Pseudomonas aeruginosa, Pseudomonas mendocina,
and Pseudomonas denitrificans are positive for deni
trification. Fluorescein production is indicated by flu
orescence under UV light. Pseudomonas aeruginosa
is positive for fluorescein production; Pseudomonas
denitrificans does not produce fluorescein.
