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Flavobacterium Medium (ATCC Medium 1687)

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]
Flavobacterium Medium
(ATCC Medium 1687)

山東拓普生物工程有限(xian)公司 培養基配(pei)方 //ljxinglong.com

Composition per liter:

Sodium glutamate ..............................................4.0g
K2HPO4............................................................0.65g
NaNO3................................................................0.5g
KH2PO4............................................................0.19g
MgSO4·7H2O .....................................................0.1g
FeSO4 solution ............................................... 2.0mL
pH 7.4 ± 0.2 at 25°C
FeSO4 Solution:
Composition per 10.0mL:
FeSO4·7H2O..................................................... 0.03g
Preparation of FeSO4 Solution: Add FeSO4 to
distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, ex
cept FeSO4 solution, to distilled/deionized water and
bring volume to 998.0mL. Mix thoroughly. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to
25°C. Aseptically add 2.0mL of sterile FeSO4 solu
tion. Mix thoroughly. Adjust pH to 7.4. Aseptically
distribute into sterile tubes or flasks.
Use: For the cultivation of Flavobacterium species.
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