培養基配方

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Enterococcosel™ Agar

[所屬分類:培養基配方] [發布時間:2021-11-18] [發布人:網站管理員2] [閱讀次數:] [返回]

山東(dong)拓(tuo)普生物(wu)工程有限公司(si) 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................17.0g

Agar ..................................................................13.5g

Oxgall................................................................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................5.0g

Peptic digest of animal tissue..............................3.0g

Esculin ................................................................1.0g

Sodium citrate.....................................................1.0g

Ferric ammonium citrate.....................................0.5g

NaN3 .................................................................0.25g

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Caution: Sodium azide is toxic. Azides also react

with metals and disposal must be highly diluted.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Pour into sterile

Petri dishes or leave in tubes.

Use: For the rapid, selective isolation, cultivation,

and enumeration of fecal group D streptococci (en

terococci). For the cultivation of staphylococci and

Listeria monocytogenes.