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Enrichment Broth, pH 7.3

[所屬分類:培養基配方] [發布時間:2021-11-17] [發布人:網站管理員2] [閱讀次數:] [返回]
Enrichment Broth, pH 7.3

山(shan)東拓(tuo)普生物(wu)工程(cheng)有限公司 培養基(ji)配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................17.0g
NaCl....................................................................5.0g
Papaic digest of soybean meal............................3.0g
Glucose ...............................................................2.5g
K2HPO4...............................................................2.5g
Yeast extract........................................................6.0g
Nalidixic acid solution.................................... 8.0mL
Cycloheximide solution .................................. 5.1mL
Acriflavin·HCl solution .................................. 2.0mL
pH 7.3 ± 0.2 at 25°C
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide....................................................0.1g
Ethanol, absolute............................................. 4.0mL
Preparation of Cycloheximide Solution: Add
components to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Nalidixic Acid Solution:
Composition per 10.0mL:
Nalidixic acid....................................................0.05g
Preparation of Nalidixic Acid Solution: Add
nalidixic acid to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Acriflavin·HCl Solution:
Composition per 10.0mL:
Acriflavin·HCl ..................................................0.05g
Preparation of Acriflavin·HCl Solution: Add
acriflavin·HCl to distilled/deionized water and bring
volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components—ex
cept cycloheximide solution, nalidixic acid solution,
and acriflavin·HCl solution—to distilled/deionized
water and bring volume to 984.9mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 5.1mL of cycloheximide solution,
8.0mL of nalidixic acid solution, and 2.0mL of acri
flavin·HCl solution. Mix thoroughly. Aseptically dis
tribute into sterile tubes.
Use: For the isolation, cultivation, and enrichment of
a variety of microorganisms from milk and dairy
products.
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