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Endo Broth (m-Endo Broth)

[所屬分類:培養基配方] [發布時間:2021-11-17] [發布人:網站管理員2] [閱讀次數:] [返回]
Endo Broth
(m-Endo Broth)

山東拓普生物工程有限公(gong)司 培養基配方 //ljxinglong.com

Composition per liter:

Lactose..............................................................12.5g
Peptone .............................................................10.0g
NaCl....................................................................5.0g
Pancreatic digest of casein..................................5.0g
Peptic digest of animal tissue .............................5.0g
K2HPO4...........................................................4.375g
Na2SO3................................................................2.1g
Yeast extract........................................................1.5g
KH2PO4...........................................................1.375g
Basic Fuchsin....................................................1.05g
Sodium deoxycholate..........................................0.1g
Ethanol (95% solution)................................. 20.0mL
pH 7.2 ± 0.1 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Caution: Basic Fuchsin is a potential carcinogen
and care must be taken to avoid inhalation of the
powdered dye and contact with the skin.
Preparation of Medium: Add ethanol to approx
imately 900.0mL of distilled/deionized water. Add
remaining components. Bring volume to 1.0L with
distilled/deionized water. Mix thoroughly. Gently
heat and bring to boiling. Rapidly cool broth below
45°C. Do not autoclave. Use 1.8–2.0mL for each fil
ter pad. Protect from the light. Prepare broth freshly.
Use: For the cultivation and enumeration of coliform
bacteria from water by the membrane filter method.
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