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Endo Agar

[所屬分類:培養基配方] [發布時間:2021-11-17] [發布人:網站管理員2] [閱讀次數:] [返回]
Endo Agar

山東拓普(pu)生物工程有限公司(si) 培(pei)養基配(pei)方 //ljxinglong.com

Composition per liter:

Agar ..................................................................10.0g
Lactose..............................................................10.0g
Peptic digest of animal tissue............................10.0g
K2HPO4...............................................................3.5g
Na2SO3................................................................2.5g
Basic Fuchsin solution.................................... 4.0mL
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from Oxoid.
Caution: Basic Fuchsin is a potential carcinogen
and care must be taken to avoid inhalation of the
powdered dye and contact with the skin.
Basic Fuchsin Solution:
Composition per 10.0mL:
Basic Fuchsin......................................................1.0g
Ethanol (95% solution) ................................. 10.0mL
Preparation of Basic Fuchsin Solution: Add
Basic Fuchsin to 10.0mL of ethanol. Mix thoroughly.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Pour into sterile Petri dishes. Swirl
flask while pouring plates to keep precipitate in sus
pension. Protect from the light.
Use: For the selective isolation, cultivation, and dif
ferentiation of coliform and other enteric microorgan
isms based on their ability to ferment lactose. Lactose
fermenting bacteria appear as dark red colonies with a
gold metallic sheen. Lactose-nonfermenting bacteria
appear as colorless or translucent colonies.
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