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培養基配方

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Egg-Yolk Tellurite Emulsion 20%

[所屬分類:培養基配方] [發布時間:2021-11-17] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養(yang)基配方 //ljxinglong.com

Composition per 100mL:

NaCl................................................................0.425g

K2TeO3 ..............................................................0.21g

Egg yolk........................................................ 20.0mL

Preparation of Medium: Use fresh eggs, less than

1 week old. Scrub the shells with soap. Let stand in a

soap solution for 30 min. Rinse in running water. Soak

eggs in 70% ethanol for 15 min. or soak eggs with

1:100 dilution of saturated mercuric chloride solution

for 1 min. Crack eggs and separate yolks from

whites, placing egg yolks into a sterile container. Use

enough eggs to produce at least 20.0mL egg yolk.

Homogenize by shaking. Add 0.45g NaCl and 0.21g

K2TeO3 to distilled/deionized water and bring vol

ume to 80.0mL. Sterilze the saline-tellurite solution

by filtration or by autoclaving for 15 min at 15 psi

pressure–121°C. If autoclaving is used, cool to 25°C.

Aseptically add 20.0mL homogenized egg yolks to

80.0mL of the sterile saline-tellurite solution. Mix

thoroughly.

Use: For use in various culture media. It may be add

ed directly to nutrient media for the identification of

Clostridium, Bacillus and Staphylococcus species by

their lipase activity.