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Egg Yolk Emulsion

[所屬分類:培養基配方] [發布時間:2021-11-17] [發布人:網站管理員2] [閱讀次數:] [返回]
Egg Yolk Emulsion

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per 100mL:

Sterile saline.................................................. 70.0mL
Egg yolk........................................................ 30.0mL
Source: Sterile egg yolk emulsion is available from
Fluka, Sigma-Aldrich.
Preparation of Medium: Use fresh eggs, less than
1 week old. Scrub the shells with soap. Let stand in a
soap solution for 30 min. Rinse in running water. Soak
eggs in 70% ethanol for 15 min or soak eggs with
1:100 dilution of saturated mercuric chloride solution
for 1 min. Crack eggs and separate yolks from
whites, placing egg yolks into a sterile container. Use
enough eggs to produce at least 30.0mL egg yolk.
Homogenize by shaking. Add 0.9g NaCl to distilled/
deionized water and bring volume to 100.0mL. Ster
ilze the saline solution by filtration or by autoclaving
for 15 min at 15 psi pressure–121°C. If autoclaving
is used, cool to 25°C. Aseptically add 30.0mL ho
mogenized egg yolks to 70.0mL of sterile saline so
lution. Mix thoroughly.
Use: Sterile stabilized emulsion of egg yolk recom
mended for use in various culture media.
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