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培養基配方
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Double-Strength Crude Medium for Lactobacillus
[所屬分類:培養基配方] [發布時間:2021-11-16] [發布人:網站管理員2] [閱讀次數:] [返回]
Double-Strength Crude Medium
for Lactobacillus
山東拓(tuo)普生物工(gong)程有限公司 培養基配方(fang) //ljxinglong.com
Composition per liter:
Sucrose..............................................................20.0g
Yeast extract......................................................20.0g
Casein hydrolysate............................................15.0g
Potassium acetate................................................3.0g
Histidine·HCl ......................................................2.0g
Ascorbic acid ......................................................1.0g
Pyridoxamine·HCl ......................................... 33.0μg
Salts A........................................................... 20.0mL
Salts B............................................................. 5.0mL
pH 5.4 ± 0.2 at 25°C
Salts A:
Composition per 100.0mL:
KH2PO4·H2O.....................................................16.5g
K2HPO4·3H2O...................................................16.5g
Preparation of Salts A: Add components to dis
tilled/deionized water and bring volume to 100.0mL.
Mix thoroughly.
Salts B:
Composition per 100.0mL:
MgSO4·7H2O ......................................................8.0g
FeSO4·7H2O........................................................0.4g
MnSO4·H2O........................................................0.4g
NaCl....................................................................0.4g
HCl, concentrated ........................................... 0.1mL
Preparation of Salts B: Add components to dis
tilled/deionized water and bring volume to 100.0mL.
Mix thoroughly.
Preparation of Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Adjust
pH to 5.4 with acetic acid. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Lacto
bacillus species.
