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Dorset Egg Medium

[所屬分類:培養基配方] [發布時間:2021-11-16] [發布人:網站管理員2] [閱讀次數:] [返回]
Dorset Egg Medium

山東(dong)拓普生物工程有限公司 培養(yang)基配方(fang) //ljxinglong.com

Composition per liter:

Homogenized whole egg ............................ 950.0mL
Glycerol ........................................................ 50.0mL
pH 6.8–7.4 at 25°C
Source: This medium is available as a prepared medi
um from BD Diagnostic Systems.
Homogenized Whole Egg:
Composition per liter:
Whole eggs ......................................................18–24
Preparation of Homogenized Whole Egg:
Use fresh eggs, less than 1 week old. Scrub the shells
with soap. Let stand in a soap solution for 30 min.
Rinse in running water. Soak eggs in 70% ethanol for 15
min. Break the eggs into a sterile container. Homoge
nize by shaking. Filter through four layers of sterile
cheesecloth into a sterile graduated cylinder. Measure
out 1.0L.
Preparation of Medium: Filter sterilize glycerol.
Combine glycerol and homogenized whole egg. Mix
thoroughly. Distribute into sterile screw-capped tubes.
Place tubes in a slanted position. Inspissate at 85°C
(moist heat) for 45 min.
Use: For the maintenance of Mycobacterium spe
cies.
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