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Differential Broth for Lactic Streptococci

[所屬分類:培養基配方] [發布時間:2021-11-16] [發布人:網站管理員2] [閱讀次數:] [返回]
Differential Broth
for Lactic Streptococci

山東拓普生物(wu)工程有限公司(si) 培養基配(pei)方 //ljxinglong.com

Composition per liter:

Sodium citrate...................................................20.0g
Arginine ..............................................................5.0g
Pancreatic digest of casein..................................5.0g
Yeast extract........................................................5.0g
K2HPO4...............................................................1.0g
Bromcresol Purple ............................................0.02g
Skim milk (11% solution)............................. 35.0mL
pH 6.2 ± 0.2 at 25°C
Preparation of Medium: Add components, except
skim milk solution, to distilled/deionized water and
bring volume to 800.0mL. Mix thoroughly. Add
35.0mL of skim milk solution. Bring volume to 1.0L
with distilled/deionized water. Place medium in a steam
bath for 15 min. Cool to 25°C. Adjust pH to 6.2. Distrib
ute 7.0mL volumes into screw-capped tubes that con
tain an inverted Durham tube. Autoclave for 15 min at
15 psi pressure–121°C. Allow autoclave to cool below
70°C before opening door.
Use: For the cultivation and differentiation of Lacto
bacillus lactis, Lactobacillus lactis subspecies cre
moris,
and
Lactobacillus lactis
subspecies
diacetylactis. Lactose-fermenting bacteria such as
Lactobacillus lactis subspecies cremoris turn the me
dium yellow. Arginine-utilizing bacteria such as Lac
tobacillus lactis initially turn the medium yellow but
then turn back to violet. Citrate-utilizing bacteria such
as Lactobacillus lactis subspecies diacetylactis turn
the medium violet and produce CO2 that is trapped as
a bubble in the Durham tube.
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