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Dextrose Tryptone Agar
[所屬分類:培養基配方] [發布時間:2021-11-16] [發布人:網站管理員2] [閱讀次數:] [返回]
Dextrose Tryptone Agar
山東拓普生(sheng)物工程有限公(gong)司 培養基配方 //ljxinglong.com
Composition per liter:
Agar ..................................................................12.0g
Pancreatic digest of casein................................10.0g
Glucose ...............................................................5.0g
Bromcresol Purple ............................................0.04g
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed pow
der from Oxoid.
Preparation of Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Distribute
into tubes or flasks. Autoclave for 15 min at 15 psi pres
sure–121°C. Pour into sterile Petri dishes or leave in
tubes.
Use: For the isolation, cultivation, and enumeration
of “flat-sour” thermophiles and mesophiles in food.
Acid-producing microorganisms such as “flat-sour”
hermophiles appear as yellow colonies surrounded
by a yellow zone.
