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Deoxycholate Citrate Agar (Desoxycholate Citrate Agar)

[所屬分類:培養基配方] [發布時間:2021-11-16] [發布人:網站管理員2] [閱讀次數:] [返回]
Deoxycholate Citrate Agar
(Desoxycholate Citrate Agar)

山東拓普生物工程有(you)限公司 培養(yang)基配方 //ljxinglong.com

Composition per liter:

Pork infusion...................................................330.0g
Sodium citrate...................................................20.0g
Agar ..................................................................13.5g
Lactose..............................................................10.0g
Proteose peptone No. 3 .....................................10.0g
Sodium deoxycholate..........................................5.0g
Ferric ammonium citrate.....................................2.0g
Neutral Red.......................................................0.02g
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a premixed pow
der from BD Diagnostic Systems.
Preparation of Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Do not au
toclave. Cool to 45°–50°C. Pour into sterile Petri dishes.
Dry the agar surface before use.
Use: For the selective isolation and cultivation of en
teric pathogens, especially Salmonella and Shigella
species.
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