99精品国产成人综合_国产不卡精品视频男人的天堂_人妻av久久一区波多野结衣_亚洲乱码卡一卡二卡新区仙踪林_人妻无码av天堂二区网站_中文字幕精品av一区二区五区_国产精品国产高清国产av_亚洲欧美日韩国产综合点击进入_亚洲欧美日韩国产制服另类_亚洲精品成av人片天堂无码

展開

編號：
批號：

培養基配方

您現在的位置：網站首頁 >> 培養基配方

CreDm1 Medium

[所屬分類:培養基配方] [發布時間:2021-11-15] [發布人:網站管理員2] [閱讀次數:] [返回]

CreDm1 Medium

山東(dong)拓(tuo)普生物工程有(you)限(xian)公司培養(yang)基配(pei)方(fang) //ljxinglong.com

Composition per 1002.0mL:

Solution A................................................... 980.0mL

Solution D..................................................... 10.0mL

Solution E ..................................................... 10.0mL

Solution B ....................................................... 1.0mL

Solution C ....................................................... 1.0mL

pH 6.7–6.9 at 25°C

Solution A:

Composition per 980.0mL:

KH2PO4...............................................................1.4g

NH4Cl .................................................................0.5g

MgCl2·6H2O .......................................................0.2g

CaCl2·2H2O ......................................................0.15g

Yeast extract...................................................50.0mg

Preparation of Solution A: Add components to

distilled/deionized water and bring volume to

980.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C.

Solution B (Trace Elements Solution SL-10):

Composition per liter:

FeCl2·4H2O.........................................................1.5g

CoCl2·6H2O .................................................190.0mg

MnCl2·4H2O ................................................100.0mg

ZnCl2..............................................................70.0mg

Na2MoO4·2H2O .............................................36.0mg

NiCl2·6H2O....................................................24.0mg

H3BO3 ..............................................................6.0mg

CuCl2·2H2O .....................................................2.0mg

HCl (25% solution)....................................... 10.0mL

Preparation of Solution B (Trace Elements

Solution SL-10): Add FeCl2·4H2O to 10.0mL of

HCl solution. Mix thoroughly. Add distilled/deion

ized water and bring volume to 1.0L. Add remaining

components. Mix thoroughly. Autoclave for 15 min

at 15 psi pressure–121°C.

Solution C (Selenite-Tungstate Solution):

Composition per liter:

NaOH..................................................................0.5g

Na2WO4·2H2O.................................................4.0mg

Na2SeO3·5H2O ................................................3.0mg

Preparation of Solution C (Selenite-Tung

state Solution): Add components to distilled/

deionized water and bring volume to 1.0L. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–

121°C.

Solution D (Vitamin Solution):

Composition per liter:

Pyridoxine·HCl ..............................................10.0mg

Calcium DL-pantothenate.................................5.0mg

Lipoic acid .......................................................5.0mg

Nicotinic acid...................................................5.0mg

p-Aminobenzoic acid.......................................5.0mg

Riboflavin ........................................................5.0mg

Thiamine·HCl ..................................................5.0mg

Biotin ...............................................................2.0mg

Folic acid..........................................................2.0mg

Vitamin B12 ......................................................0.1mg

Preparation of Solution D (Vitamin Solution):

Add components to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Filter steril

ize.

Solution E:

Composition per 10.0mL:

Disodium-DL-malate ...........................................1.6g

Preparation of Solution E: Add disodium-DL

malate to distilled/deionized water and bring volume

to 10.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C.

Preparation of Medium: Aseptically combine

980.0mL of sterile solution A with 1.0mL of sterile

solution B, 1.0mL of sterile solution C, 10.0mL of

sterile solution D, and 10.0mL of sterile solution E,

in that order. Mix thoroughly. Adjust pH to 6.7–6.9.

Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Campylobacter species.

<bdo id='aG5p0'><sup id='2g9HB'><div id='sgJEc'><bdo id='Q1kjh'></bdo></div></sup></bdo>

在線客服

CreDm1 Medium