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Cornmeal and V8 Juice Agar (ATCC Medium 309)

[所屬分類:培養基配方] [發布時間:2021-11-15] [發布人:網站管理員2] [閱讀次數:] [返回]
Cornmeal and V8 Juice Agar
(ATCC Medium 309)

山東(dong)拓(tuo)普生物工程(cheng)有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ....................................................................7.5g
CaCO3 .................................................................3.0g
Cornmeal Extract ........................................ 800.0mL
V8 Juice ...................................................... 200.0mL
pH 5.6–6.0 at 25°C
Cornmeal Extract:
Composition per 800.0mL:
Yellow cornmeal ...............................................50.0g
Preparation of Cornmeal Infusion: Add 50.0g of
yellow Cornmeal to 800.0mL of water. Leave in re
frigerator overnight. Heat Cornmeal mixture to 60°C
for 1 hr. Filter out Cornmeal through cheese cloth.
Bring volume back to 800.0mL.
Preparation of Medium: Combine components.
Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of fungi.
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