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Congo Red Magnesium Oxalate Agar (CRMOX Agar)

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]
Congo Red Magnesium
Oxalate Agar
(CRMOX Agar)

山東拓(tuo)普生(sheng)物工程有限公(gong)司 培養基配方 //ljxinglong.com

Composition per liter:

Solution 1.................................................... 825.0mL
Solution 2...................................................... 80.0mL
Solution 3...................................................... 80.0mL
Solution 4...................................................... 10.0mL
Solution 5........................................................ 5.0mL
pH 7.3 ± 0.2 at 25°C
Solution 1:
Composition per 825.0mL:
Pancreatic digest of casein................................15.0g
Agar ..................................................................15.0g
Papaic digest of soybean meal............................5.0g
NaCl....................................................................5.0g
pH 7.3 ± 0.2 at 25°C
Preparation of Solution 1: Add components to
distilled/deionized water and bring volume to
825.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Do not overheat.
Solution 2:
Composition per liter:
MgCl2·6H2O .....................................................50.8g
Preparation of Solution 2: Add MgCl2·6H2O to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Autoclave for 15 min at 15 psi pres
sure–121°C.
Solution 3:
Composition per liter:
Sodium oxalate .................................................33.2g
Preparation of Solution 3: Add sodium oxalate
to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C.
Solution 4:
Composition per 100.0mL:
D-Galactose .......................................................20.0g
Preparation of Solution 4: Add D-galactose to
distilled/deionized water and bring volume to
100.0mL. Mix thoroughly. Filter sterilize.
Solution 5:
Composition per 10.0mL:
Congo Red ..........................................................0.1g
Preparation of Solution 5: Add Congo Red to
distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Autoclave for 15 min at 15
psi pressure–121°C.
Preparation of Medium: Aseptically combine
80.0mL of sterile solution 2, 80.0mL of sterile solu
tion 3, 10.0mL of sterile solution 4, and 5.0mL of
sterile solution 5. Mix thoroughly. Warm to 50°C.
Add this mixture to 825.0mL of cooled, sterile solu
tion 1. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the cultivation and identification of patho
genic serotypes of Yersinia enterocolitica. For the de
termination of whether Yersinia strains contain the
Yersinia virulence plasmid.
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