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Coliform Medium (CM)

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]

Coliform Medium

(CM)

山東拓普生(sheng)物工(gong)程有限(xian)公司培養基配方 //ljxinglong.com

Composition per liter:

Bile salts No. 3..................................................20.0g

Lactose..............................................................20.0g

Proteose peptone No. 3.....................................10.0g

Yeast extract........................................................6.0g

Sodium lauryl sulfate..........................................1.0g

Sodium deoxycholate..........................................0.1g

Bromcresol Purple solution .......................... 10.0mL

pH 6.8 ± 0.2 at 25°C

Bromcresol Purple Solution:

Composition per 100.0mL:

Bromcresol Purple ............................................0.35g

NaOH (0.1N solution)..................................... 2.0mL

Preparation of Bromcresol Purple Solution:

Combine Bromcresol Purple and NaOH solution.

Mix thoroughly. Bring volume to 100.0mL with dis

tilled/deionized water. Filter sterilize.

Preparation of Medium: Add components, ex

cept Bromcresol Purple solution, to distilled/deion

ized water and bring volume to 990.0mL. Mix

thoroughly. Add 10.0mL of Bromcresol Purple solu

tion. Mix thoroughly. Adjust pH to 6.8 with 1N

NH4OH. Distribute into flasks in 95.0mL volumes.

Autoclave for 25 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of coliform mi

croorganisms from yogurt and raw milk.