99精品国产成人综合_国产不卡精品视频男人的天堂_人妻av久久一区波多野结衣_亚洲乱码卡一卡二卡新区仙踪林_人妻无码av天堂二区网站_中文字幕精品av一区二区五区_国产精品国产高清国产av_亚洲欧美日韩国产综合点击进入_亚洲欧美日韩国产制服另类_亚洲精品成av人片天堂无码

展開
個人中心 我的訂單 購物車
編號：
批號：
培養基配方
您現在的位置： 網站首頁 >> 培養基配方

Coliform Agar ES, Chromocult (Chromocult Coliform Agar ES) (Chromocult Enhanced Selectivity Agar)

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]
Coliform Agar ES, Chromocult
(Chromocult Coliform Agar ES)
(Chromocult Enhanced Selectivity
Agar)

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................10.0g
MOPS ...............................................................10.0g
KCl......................................................................7.5g
Peptone ..............................................................5.0g
Bile salts............................................................1.15g
Na-propionate .....................................................0.5g
6-Chloro-3-indoxyl-β-D-galactopyranoside ....0.15g
5-Bromo-4-chloro-3-indoxyl-
β-D-glucuronic acid .....................................0.1g
Isopropyl-β-D-thiogalactopyranoside ................0.1g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available from Merck.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly and heat with frequent agitation until
components are completely dissolved (approximate
ly 45 min). Do not autoclave. Cool to 45°–50°C.
Pour into sterile Petri dishes. The plates should be
clear and colorless.
Use: For the detection of E. coli and total coliforms.
The combination of suitable peptones and the buffer
ing using MOPS allow rapid growth of coliforms and
an optimal transformation of the chromogenic sub
strates. The amount of bile salts and propionate large
ly inhibit growth of Gram-positive and Gram-nega
tive accompanying flora. The simultaneous detection
of total coliforms and E. coli is achieved using the
combination of two chromogrenic substrates. The
substrate Salmon™-β-D-GAL is split by β-D-galac
tosidase, characteristic for coliforms, resulting in a
salmon to red coloration of coliform colonies. The
detection of the β-D-glucuronidase, characteristic for
E. coli, is cleaved via the substrate X-β-D-glucu
ronide, causing a blue coloration of positive colonies.
As E. coli splits Salmon-β-D-GAL as well as X-β-D
glucuronide, the colonies turn to a dark violet color
and can be easily differentiated from the other
coliforms being salmon-red.
  版權所有：山東拓普生物工程有限公司 營銷服務熱線：　郵箱：
© 2016-2020 ALL RIGHTS RESERVED.　　
購物車 去頂部