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Coletsos Medium

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]
Coletsos Medium

山東拓普生物工程有限(xian)公司 培養基(ji)配方 //ljxinglong.com

Composition per 1625mL:

Potato starch......................................................10.0g
Gelatin.................................................................4.0g
Asparagine ........................................................2.25g
KH2PO4...............................................................1.5g
Na-glutamate.......................................................1.0g
Na-pyruvate ........................................................1.0g
Mg-citrate........................................................0.375g
Litmus ...............................................................0.25g
Malachite green.................................................0.25g
MgSO4 ..............................................................0.15g
Activated carbon .................................................0.1g
Oligonucleotide mixture ..................................3.0mg
Homogenized egg ...................................... 625.0mL
Glycerol .......................................................... 7.5mL
Egg Mixture:
Composition per liter:
Whole eggs ......................................................18–24
Preparation of Egg Mixture: Use fresh eggs,
less than 1 week old. Scrub the shells with soap. Let
stand in a soap solution for 30 min. Rinse in running
water. Soak eggs in 70% ethanol for 15 min. Break
the eggs into a sterile container. Separate egg whites
from egg yolks. Combine 8 parts egg white with 2
parts egg yolk. Homogenize by shaking. Filter
through four layers of sterile cheesecloth into a ster
ile graduated cylinder. Measure out 1.0L.
Preparation of Medium: Add glycerol to
600.0mL of distilled/deionized water. Mix thorough
ly. Add remaining components, except egg mixture.
Bring volume to 1.0L. Mix thoroughly. Gently heat
while stirring and bring to boiling. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 50°C. Asepti
cally add 625.0mL of egg mixture. Mix thoroughly.
Distribute into sterile screw-capped tubes. Place
tubes in a slanted position. Inspissate at 85°C (moist
heat) for 45 min.
Use: For the cultivation of Mycobacterium tuberculo
sis.
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