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Clostridium CK Medium (DSMZ Medium 869)

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]
Clostridium CK Medium
(DSMZ Medium 869)

山(shan)東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein..................................3.2g
NaCl....................................................................0.9g
Papaic digest of soybean meal............................0.6g
K2HPO4...............................................................0.5g
Glucose ...............................................................0.5g
Resazurin .........................................................0.5mg
Glucose solution ........................................... 10.0mL
pH 5.5 ± 0.2 at 25°C
Glucose Solution:
Composition per 10.0mL:
Glucose..............................................................2.0g
Preparation of Glucose Solution: Add glucose
to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Sparge with 100% N2. Fil
ter sterilize.
Preparation of Medium: Add components, ex
cept glucose solution, to distilled/deionized water
and bring volume to 990.0mL. Mix thoroughly. Ad
just pH to 5.5. Sparge with 100% N2. Autoclave for
15 min at 15 psi pressure–121°C. Cool to 25°C.
Aseptically and anaerobically add 10.0mL sterile
glucose solution. Aseptically and anaerobically dis
tribute into tubes or flasks.
Use: For the cultivation of Clostridium akagii,
Clostridium acidisoli, and Clostridium uliginosum.
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