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Clostridium acidurici Medium

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]
Clostridium acidurici Medium

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Uric acid..............................................................2.0g
Yeast extract........................................................1.0g
K2HPO4.............................................................0.91g
KOH..................................................................0.67g
Sodium thioglycolate..........................................0.5g
MgSO4·7H2O....................................................0.25g
CaCl2·2H2O ....................................................0.015g
FeSO4·7H2O.....................................................6.0mg
NaHCO3 solution .......................................... 25.0mL
Sodium thioglycolate solution ...................... 25.0mL
pH 7.0–7.5 at 25°C
NaHCO3 Solution:
Composition per 25.0mL:
NaHCO3..............................................................5.0g
Preparation of NaHCO3 Solution: Add
NaHCO3 to distilled/deionized water and bring vol
ume to 25.0mL. Mix thoroughly. Filter sterilize.
Sodium Thioglycolate Solution:
Composition per 25.0mL:
Sodium thioglycolate..........................................0.5g
Preparation of Sodium Thioglycolate Solu
tion: Add sodium thioglycolate to distilled/deion
ized water and bring volume to 25.0mL. Mix
thoroughly. Autoclave solution separately for 15 min
at 15 psi pressure–121°C.
Preparation of Medium: Add K2HPO4 and KOH
to distilled/deionized water and bring volume to 1.0L.
Add uric acid. Gently heat until boiling. Add the re
maining components, except NaHCO3 and sodium
thioglycolate. Mix thoroughly. Adjust pH to 7.0–7.5.
Distribute into tubes or flasks. Autoclave for 15 min at
15 psi pressure–121°C. Add 0.25mL of sterile
NaHCO3 solution and 0.25mL of sterile sodium
thioglycolate solution for each 10.0mL of sterile basal
medium.
Use: For the cultivation and maintenance of
Clostridium acidurici, Clostridium purinolyticum,
and other bacteria that can utilize uric acid as a car
bon source.
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