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培養基配方

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Chromogenic Salmonella Esterase Agar (CSE Agar)

[所屬分類:培養基配方] [發布時間:2021-11-12] [發布人:網站管理員2] [閱讀次數:] [返回]

Chromogenic Salmonella Esterase Agar

(CSE Agar)

山(shan)東拓普生物工(gong)程有(you)限(xian)公司培養基配方(fang) //ljxinglong.com

Composition per liter:

Lactose..............................................................14.65

Agar ..................................................................12.0g

Peptone................................................................4.0g

Tryptone..............................................................4.0g

Tween 20.............................................................3.0g

Lab Lemco..........................................................3.0g

Na3-citrate dihydrate...........................................0.5g

L-cysteine........................................................0.128g

Tris....................................................................0.06g

SLA-octonoate solution................................ 50.0mL

Novobiocin solution...................................... 10.0mL

Ethyl 4-dimethylaminobenzoate solution..... 10.0mL

pH 7.0 ± 0.2 at 25°C

Novobiocin Solution:

Composition per 10mL:

Novobiocin ....................................................70.0mg

Preparation of Novobiocin Solution: Add no

vobiocin to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Ethyl 4-dimethylaminobenzoate Solution:

Composition per 10mL:

Ethyl 4-dimethylaminobenzoate.......................0.35g

Methanol......................................................... 8.0mL

Preparation of Ethyl 4-dimethylaminoben

zoate Solution: Add ethyl 4-dimethylaminoben

zoate to 8.0mL methanol. Mix thorougly. Bring

volume to 10.0mL with distilled/deionized water.

Mix thoroughly. Filter sterilize.

SLA-Octonoate Solution:

Composition per 50mL:

4-[2-(4-octanoyloxy-3,5-dimethoxyphenyl)-

vinyl]-quinolinium-1-(propan-3-yl

carboxylic acid) bromide

(SLPA-octanoate; bromide form)...........0.3223g

Preparation of SLA-Octonoate Solution: Add

SLA-octonoate to distilled/deionized water and bring

volume to 50.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept novobiocin solution, SLA-octonoate solution,

and ethyl 4-dimethylaminobenzoate solution, to dis

tilled/deionized water and bring volume to 920.0mL.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°C. Aseptically add 10.0mL novobiocin solu

tion, 50.0mL SLA-octonoate solution, and 10.0mL

ethyl 4-dimethylaminobenzoate solution. Mix thor

oughly. Pour into sterile Petri dishes.

Use: For the differntiation of Salmonella spp.

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Chromogenic Salmonella Esterase Agar (CSE Agar)