培養基配方

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Chromogenic Bacillus cereus Agar

[所屬分類:培養基配方] [發布時間:2021-11-11] [發布人:網站管理員2] [閱讀次數:] [返回]

山東(dong)拓(tuo)普生物工程有限公司培養(yang)基配方 //ljxinglong.com

Composition per liter:

Agar ..................................................................13.0g

Peptone..............................................................10.0g

Sodium pyruvate...............................................10.0g

Yeast extract........................................................4.0g

Na2HPO4 .............................................................2.52

Chromogenic mix ...............................................1.2g

KH2PO4.............................................................0.28g

Selective supplement solution ...................... 10.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Selective Supplement Solution:

Composition per 10.0mL:

Trimethoprim.................................................10.0mg

Polymyxin B sulfate ..................................106,000U

Preparation of Selective Supplement Solu

tion: Add componetns to distilled/deionized water

and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except

selective supplement solution, to distilled/deionized

water and bring volume to 990.0mL. Mix thoroughly.

Gently heat and bring to boiling. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 50°C. Aseptically add

10.0mL selective supplement solution. Mix thoroughly.

Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: A chromogenic medium for the isolation and

differentiation of Bacillus cereus from food samples.