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培養基配方
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Christensen Citrate Agar (BAM M39)
[所屬分類:培養基配方] [發布時間:2021-11-11] [發布人:網站管理員2] [閱讀次數:] [返回]
Christensen Citrate Agar
(BAM M39)
山東拓普生物工程有限公司 培養基配方(fang) //ljxinglong.com
Composition per liter:
Agar ..................................................................15.0g
NaCl....................................................................5.0g
Sodium citrate.....................................................3.0g
KH2PO4...............................................................1.0g
Yeast extract........................................................0.5g
Ferric ammonium citrate.....................................0.4g
L-Cysteine·HCl·H2O ...........................................0.1g
Na2S2O5 ............................................................0.08g
Phenol Red.....................................................12.0mg
pH 6.9 ± 0.2 at 25°C
Preparation of Medium: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Gently heat and bring to boiling. Dispense
into tubes or flasks. Autoclave for 15 min at 15 psi
pressure–121°C. Pour into sterile Petri dishes or leave
in tubes. Allow tubes to cool in a slanted position.
Use: For the differentiation of enteric pathogens, es
pecially members of the Enterobacteriaceae, and
coliforms based on their ability to utilize citrate as a
carbon source. Bacteria that can utilize citrate turn
the medium pink-red.
