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Cheese Agar

[所屬分類:培養基配方] [發布時間:2021-11-11] [發布人:網站管理員2] [閱讀次數:] [返回]
Cheese Agar

山東拓(tuo)普生物工程有(you)限(xian)公(gong)司 培養基配方 //ljxinglong.com

Composition per liter:

Cheese, ripened...............................................100.0g
NaCl..................................................................50.0g
Agar ..................................................................15.0g
Peptone..............................................................10.0g
Potassium citrate...............................................10.0g
Sodium oxalate ...................................................2.0g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add the cheese and po
tassium citrate to distilled/deionized water and bring
volume to 300.0mL. Gently heat and bring to 50°C to
separate the fat. Discard the fat. In a separate flask,
add the remaining components to distilled/deionized
water and bring volume to 700.0mL. Gently heat and
bring to boiling. Add the 300.0mL of aqueous sus
pension of cheese solids. Adjust pH to 7.4. Autoclave
for 25 min at 15 psi pressure–121°C. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Brevibacte
rium linens.
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