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培養基配方
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Cell Growth Medium (BAM M36)
[所屬分類:培養基配方] [發布時間:2021-11-11] [發布人:網站管理員2] [閱讀次數:] [返回]
Cell Growth Medium
(BAM M36)
山東拓普生物工程有限公司 培養基配方 //ljxinglong.com
Composition per 2250.0mL:
Eagle’s minimal essential medium
with Hanks’ salts (MEMH) ......................... 1.0L
L 15 medium, modified Leibovitz..................... 1.0L
Fetal calf serum........................................... 200.0mL
NaHCO3 solution.......................................... 50.0mL
pH 7.5 at 25°C
MEMH:
Composition per liter:
NaCl....................................................................8.0g
Glucose ...............................................................1.0g
KCl......................................................................0.4g
CaCl2·2H2O ......................................................0.14g
MgSO4·7H2O......................................................0.1g
KH2PO4.............................................................0.06g
Na2HPO4...........................................................0.05g
L-Isoleucine.....................................................0.026g
L-Leucine ........................................................0.026g
L-Lysine...........................................................0.026g
L-Threonine.....................................................0.024g
L-Valine .........................................................0.0235g
L-Tyrosine .......................................................0.018g
L-Arginine .....................................................0.0174g
L-Phenylalanine.............................................0.0165g
L-Cystine .........................................................0.012g
L-Histidine........................................................8.0mg
L-Methionine....................................................7.5mg
Phenol Red.......................................................5.0mg
L-Tryptophan....................................................4.0mg
Inositol .............................................................1.8mg
Biotin ...............................................................1.0mg
Folic acid .........................................................1.0mg
Calcium pantothenate ......................................1.0mg
Choline chloride...............................................1.0mg
Nicotinamide....................................................1.0mg
Pyridoxal·HCl ..................................................1.0mg
Thiamine·HCl ..................................................1.0mg
Riboflavin ........................................................0.1mg
Preparation of MEMH: Add components to dis
tilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Filter sterilize.
L 15 Medium, Modified Leibovitz
Composition per liter:
NaCl....................................................................8.0g
DL-Threonine.......................................................0.6g
Sodium pyruvate.................................................0.6g
DL-Alanine...........................................................0.5g
L-Arginine, free base ...........................................0.5g
KCl......................................................................0.4g
L-Asparagine·H2O ...............................................0.3g
L-Histidine, free base...........................................0.3g
L-Glutamine.........................................................0.3g
L-Isoleucine .........................................................0.3g
L-Phenylalanine...................................................0.3g
L-Tyrosine............................................................0.3g
DL-Methionine.....................................................0.2g
DL-Valine .............................................................0.2g
Glycine................................................................0.2g
L-Serine ...............................................................0.2g
Na2HPO4, anhydrous ..........................................0.2g
CaCl2, anhydrous ................................................0.1g
L-Cysteine, free base ...........................................0.1g
L-Leucine·HCl.....................................................0.1g
Streptomycin.......................................................0.1g
MgCl2, anhydrous ...........................................0.094g
D-Galactose .......................................................0.09g
KH2PO4.............................................................0.06g
Gentamicin........................................................0.05g
L-Tryptophan.....................................................0.02g
Phenol Red........................................................0.01g
i-Inositol...........................................................2.0mg
Choline chloride...............................................1.0mg
D-Calcium pantothenate ...................................1.0mg
Folic acid..........................................................1.0mg
Nicotinamide....................................................1.0mg
Pyridoxine·HCl ................................................1.0mg
Thiamine monophosphate·2H2O......................1.0mg
Riboflavin-5-phosphate ...................................0.1mg
Penicillin G ................................................100,000U
pH 7.5 ± 0.2 at 25°C
Preparation of L 15 Medium, Modified Lei
bovitz: Add components to distilled/deionized wa
ter and bring volume to 1.0L. Mix thoroughly. Filter
sterilize. Store at 5°C.
NaHCO3 Solution:
Composition per 100.0mL:
NaHCO3 ..............................................................7.5g
Preparation of NaHCO3 Solution: Add
NaHCO3 to distilled/deionized water and bring vol
ume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Aseptically combine
1.0L of sterile Eagle’s minimal essential medium
with Hanks’ salts (MEMH) and 1.0L of sterile L 15
medium, modified Leibovitz. Mix thoroughly. Im
mediately prior to use, aseptically add 200.0mL of
fetal calf serum and 50.0mL of NaHCO3 solution.
Mix thoroughly. Aseptically distribute into sterile
containers.
Use: For the cultivation of mammalian HeLa or Vero
tissue culture cells to test the cytopathic effects of Es
cherichia coli.
