培養基配方

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Campylobacter fetus Medium

[所屬分類:培養基配方] [發布時間:2021-11-10] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有(you)限公司(si) 培養基配方 //ljxinglong.com

Composition per 1160.0mL:

Fluid thioglycolate agar ..................................... 1.0L

Sheep blood, defibrinated ........................... 150.0mL

Antibiotic solution ........................................ 10.0mL

pH 7.1 ± 0.2 at 25°C

Fluid Thioglycolate Agar:

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of casein................................15.0g

Glucose ...............................................................5.5g

Yeast extract........................................................5.0g

NaCl....................................................................2.5g

Agar ..................................................................0.75g

L-Cystine .............................................................0.5g

Sodium thioglycolate..........................................0.5g

Resazurin .........................................................1.0mg

Preparation of Fluid Thioglycolate Agar:

Add components to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 25°C.

Antibiotic Solution:

Composition per 10.0mL:

Cycloheximide..................................................0.05g

Novobiocin ......................................................5.0mg

Bacitracin.....................................................25,000U

Polymyxin B sulfate ....................................10,000U

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 1.0L of cooled, ster

ile, fluid thioglycolate agar, aseptically add 150.0mL

of sterile sheep blood and 10.0mL of sterile antibiotic

solution.

Use: For the isolation and cultivation of Campylo

bacter fetus from human specimens.