培養基配方

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Campylobacter fecalis Medium

[所屬分類:培養基配方] [發布時間:2021-11-10] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per 1133.6mL:

Starch, soluble.....................................................2.4g

Yeast extract........................................................2.4g

Blood agar base.................................................. 1.0L

Bovine blood............................................... 120.0mL

Antibiotic solution ........................................ 10.0mL

Sodium lactate (60% syrup)............................ 3.6mL

pH 6.8 ± 0.2 at 25°C

Blood Agar Base:

Composition per liter:

Beef heart, solids from infusion......................500.0g

Agar ..................................................................15.0g

Tryptose ............................................................10.0g

NaCl....................................................................5.0g

Preparation of Blood Agar Base: Add compo

nents to distilled/deionized water and bring volume

to 1.0L. Mix thoroughly. Gently heat while stirring

and bring to boiling.

Antibiotic Solution:

Composition per 10.0mL:

Cycloheximide..................................................0.12g

Albamycin........................................................6.0mg

Bacitracin........................................................6000U

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: To 1.0L of blood agar

base, add soluble starch, yeast extract, and sodium

lactate. Mix thoroughly. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add sterile bovine blood and antibiotic solution. Mix

thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the cultivation and isolation of Campylo

bacter fecalis.