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Campylobacter Blood-Free Agar Base Modified (CCDA, Modified)

[所屬分類:培養基配方] [發布時間:2021-11-10] [發布人:網站管理員2] [閱讀次數:] [返回]

Campylobacter Blood-Free Agar

Base Modified

(CCDA, Modified)

山東拓普生物工程有限公司培養基配方 //ljxinglong.com

Composition per liter:

Peptone..............................................................20.0g

Agar ..................................................................12.0g

NaCl....................................................................5.0g

Activated charcoal ..............................................4.0g

Casein hydrolysate..............................................3.0g

Na-deoxycholate .................................................1.0g

Na-pyruvate ......................................................0.25g

FeSO4 ................................................................0.25g

Cefaperazone-amhotericin B solution .......... 10.0mL

pH 7.4 ± 0.2 at 25°C

Cefoperazone-Amphotericin B Solution:

Composition per 10.0mL:

Cefoperazone ..................................................0.016g

Amphotericn B................................................0.005g

Preparation of Cefoperazone-Amphotericin

B Solution: Add cefoperazone and amphotericin B

to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components ex

cept cefaperazone-amphotericin B solution to dis

tilled/deionized water and bring volume to 990.0mL.

Mix thoroughly. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Aseptically add

10.0mL of cefoperazone-amphotericin B solution.

Mix thoroughly. Pour into sterile Petri dishes or asep

tically distribute into tubes or flasks.

Use: For the isolation of Campylobacter spp. from

foods. The use of Campylobacter Blood-Free Selec

tive Agar is specified by the UK Ministry of Agricul

ture, Fisheries and Food (MAFF) in a validated meth

od for isolation of Campylobacter from foods. Am

photericin largely reduces the growth of yeasts and

molds. Cefoperazone especially inhibits Enterobac

teriaceae.