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培養基配方
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Campylobacter Agar, Blaser’s (Blaser’s Campylobacter Agar)
[所屬分類:培養基配方] [發布時間:2021-11-10] [發布人:網站管理員2] [閱讀次數:] [返回]
Campylobacter Agar, Blaser’s
(Blaser’s Campylobacter Agar)
山東拓普(pu)生物(wu)工程有限公(gong)司 培養基配(pei)方(fang) //ljxinglong.com
Composition per liter:
Campylobacter agar base............................ 990.0mL
Supplement B................................................ 10.0mL
pH 7.4 ± 0.2 at 25°C
Campylobacter Agar Base:
Composition per liter:
Proteose peptone...............................................15.0g
Agar ..................................................................12.0g
NaCl....................................................................5.0g
Yeast extract........................................................5.0g
Liver digest .........................................................2.5g
Source: Campylobacter agar base and Campylo
bacter antimicrobic supplement B are available as a
premixed powder from BD Diagnostic Systems.
Preparation of Campylobacter Agar Base:
Add components to distilled/deionized water and
bring volume to 990.0mL. Mix thoroughly. Gently
heat and bring to boiling. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C.
Supplement B:
Composition per 10.0mL:
Cephalothin....................................................15.0mg
Vancomycin ...................................................10.0mg
Trimethoprim...................................................5.0mg
Amphotericin B ...............................................2.0mg
Polymyxin B ...................................................2500U
Preparation of Supplement B: Add components
to 10.0mL of distilled/deionized water. Filter sterilize.
Preparation of Medium: Prepare 990.0mL of
Campylobacter agar base. Autoclave and cool to 45°–
50°C. Aseptically add 10.0mL of sterile supplement
B. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the selective isolation of Campylobacterje
juni from foods.
