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Brucella Semisolid Medium with Cysteine

[所屬分類:培養基配方] [發布時間:2021-11-9] [發布人:網站管理員2] [閱讀次數:] [返回]

Brucella Semisolid Medium

with Cysteine

山東(dong)拓普生物工程有限公司培(pei)養基配方 //ljxinglong.com

Composition per liter:

Peptamin ...........................................................10.0g

Pancreatic digest of casein................................10.0g

Glycine..............................................................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................2.0g

Agar ....................................................................1.8g

Glucose ...............................................................1.0g

L-Cysteine·HCl·H2O ...........................................0.2g

NaHSO3 ..............................................................0.1g

Sodium citrate.....................................................0.1g

Neutral Red solution..................................... 10.0mL

pH 7.0 ± 0.2 at 25°C

Neutral Red Solution:

Composition per 100.0mL:

Neutral Red.........................................................0.2g

Ethanol.......................................................... 10.0mL

Preparation of Neutral Red Solution: Add

Neutral Red to 10.0mL of ethanol. Bring volume to

100.0mL.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes in 10.0mL volumes. Autoclave

for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and differentiation of

Campylobacter species based on H2S production

from cysteine.