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Brucella Anaerobic Blood Agar

[所屬分類:培養基配方] [發布時間:2021-11-9] [發布人:網站管理員2] [閱讀次數:] [返回]
Brucella Anaerobic Blood Agar

山東拓普生物工程有限公司 培養基配方 //ljxinglong.com

Composition per liter:

Vitamin K1 ........................................................0.01g
Anaerobic agar base.................................... 950.0mL
Sheep blood, sterile, defibrinated ................. 50.0mL
Anaerobic Agar Base
Composition per liter:
Pancreatic digest of casein................................17.5g
Agar ..................................................................15.0g
Glucose .............................................................10.0g
Papaic digest of soybean meal............................2.5g
NaCl....................................................................2.5g
Sodium thioglycolate ..........................................2.0g
Sodium formaldehyde sulfoxylate......................1.0g
L-Cystine·HCl·H2O .............................................0.4g
Methylene Blue...............................................0.002g
pH 7.0 ± 0.2 at 25°C
Preparation of Anaerobic Agar Base: Add
components to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 45°–50°C.
Preparation of Medium: To 950.0mL of cooled,
sterile anaerobic agar base, aseptically add 10.0mg of
vitamin K1 and 50.0mL of sterile, defibrinated sheep
blood.
Use: For the isolation of anaerobes.
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