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Brucella Albimi Broth with 0.16% Agar

[所屬分類:培養基配方] [發布時間:2021-11-9] [發布人:網站管理員2] [閱讀次數:] [返回]

山東(dong)拓普生物(wu)工(gong)程有限(xian)公司(si) 培養基配方 //ljxinglong.com

Composition per liter:

Pancreatic digest of casein................................10.0g

Peptic digest of animal tissue ...........................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................2.0g

Agar ....................................................................1.6g

Glucose ...............................................................1.0g

NaHSO3 ..............................................................0.1g

Horse blood, defibrinated ........................... 100.0mL

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components, ex

cept horse blood, to distilled/deionized water and

bring volume to 900.0mL. Mix thoroughly. Heat gen

tly with frequent mixing. Boil for 1 min. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 45°–

50°C. Aseptically add 100.0mL of sterile defibrinat

ed horse blood. Mix thoroughly. Aseptically distrib

ute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Campy

lobacter species.

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