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Brilliant Green Bile Broth with MUG

[所屬分類:培養基配方] [發布時間:2021-11-9] [發布人:網站管理員2] [閱讀次數:] [返回]

山東拓普生物(wu)工程有限公司培養基配(pei)方(fang) //ljxinglong.com

Composition per liter:

Oxgall, dehydrated............................................20.0g

Lactose..............................................................10.0g

Pancreatic digest of gelatin...............................10.0g

MUG (4-Methyl umbelliferyl-

β-D-glucuronide).........................................0.05g

Brilliant Green ................................................0.013g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes containing in

verted Durham tubes, in 10.0mL amounts for testing

1.0mL or less of sample. Autoclave for 12 min (not

longer than 15 min) at 15 psi pressure–121°C. After

sterilization, cool the broth rapidly.

Use: For the detection of coliform microorganisms

in foods, dairy products, water, and wastewater, as

well as in other materials of sanitary importance. The

presence of Eschrichia coli and other coliforms is de

termined by the presence of fluorescence in the tube.

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